Open AccessA novel neisserial shuttle plasmid: A useful new tool for meningococcal research
Author(s) -
O' Dwyer Clíona A.,
Langford Paul R.,
Kroll J. Simon
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.07.036
Subject(s) - plasmid , acinetobacter baumannii , shuttle vector , microbiology and biotechnology , neisseria meningitidis , biology , neisseriaceae , escherichia coli , acinetobacter , neisseria , kanamycin , bacteria , genetics , recombinant dna , pseudomonas aeruginosa , gene , vector (molecular biology) , antibiotics
We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram‐negative bacterium Neisseria flavescens . The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas . Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis , and can be used as a shuttle plasmid in meningococcal research.