A novel neisserial shuttle plasmid: A useful new tool for meningococcal research | Zendy

O' Dwyer Clíona A. | Zendy; Langford Paul R. | Zendy; Kroll J. Simon | Zendy

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A novel neisserial shuttle plasmid: A useful new tool for meningococcal research

Author(s) -

O' Dwyer Clíona A.,

Langford Paul R.,

Kroll J. Simon

Publication year - 2005

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2005.07.036

Subject(s) - plasmid , acinetobacter baumannii , shuttle vector , microbiology and biotechnology , neisseria meningitidis , biology , neisseriaceae , acinetobacter , escherichia coli , neisseria , kanamycin , bacteria , genetics , recombinant dna , pseudomonas aeruginosa , gene , antibiotics , vector (molecular biology)

We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram‐negative bacterium Neisseria flavescens . The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas . Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis , and can be used as a shuttle plasmid in meningococcal research.

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