Open AccessRole of the 5′‐UTR in accumulation of the rbpA1 transcript at low temperature in the cyanobacterium Anabaena variabilis M3
Author(s) -
Ehira Shigeki,
Ohmori Masayuki,
Sato Naoki
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.07.034
Subject(s) - anabaena variabilis , transcription (linguistics) , biology , gene , rna , untranslated region , enhancer , messenger rna , coding region , gene expression , rna binding protein , rna recognition motif , microbiology and biotechnology , genetics , cyanobacteria , linguistics , philosophy , bacteria
The expression of the rbp genes, which encode RNA‐binding proteins with a single RNA‐recognition motif and a glycine‐rich sequence, is known to increase at low temperature in cyanobacteria. We previously showed that their regulation involved both transcription and mRNA stability. In the present study, various reporter constructs with deletions and mutations were used to analyze this regulation, revealing that at least the following three elements are involved. First, a putative enhancer element is located within the upstream gene. Second, the rbpA1 transcript is dramatically stabilized by a large stem‐loop structure located at the 5′ terminus. Third, the transcript is also destabilized by a downstream box located within the coding region.