Open AccessInfluence of nutrition on the production and physiology of sectors produced by the insect pathogenic fungus Metarhizium anisopliae
Author(s) -
Shah Farooq A.,
Butt Tariq M.
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.07.011
Subject(s) - metarhizium anisopliae , biology , esterase , virulence , microbiology and biotechnology , fungus , metarhizium , nutrient , strain (injury) , insect , enzyme , food science , botany , biological pest control , gene , genetics , biochemistry , ecology , anatomy
Metarhizium anisopliae strains V245 and V275 differed in their stability when grown on different nutrient media. V275 produced fewer sectors than V245 irrespective of the cultural conditions. Both strains produced more sectors on nutrient rich media. At least four distinct types of sectors were produced in vitro. Most sectors were sterile or sporulated poorly and produced significantly lower quantities of virulence determining enzymes like Pr1. Real‐time PCR confirmed differential expression of the pathogenicity‐related genes pr1 A , ste 1 , try 1 , and chy 1 encoding for the subtilisin Pr1A, esterase, trypsin and chymotrypsin, respectively. API‐ZYM revealed that the enzyme profiles of sectors differed from those of the parent cultures and also from other sectors. Sectors of M. anisopliae also produced less destruxins than the parent cultures independent of the strain.