Open AccessPeptidoglycan precursor pools associated with MraY and FtsW deficiencies or antibiotic treatments
Author(s) -
Lara Beatriz,
MenginLecreulx Dominique,
Ayala Juan A.,
Heijenoort Jean
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.07.005
Subject(s) - peptidoglycan , pentapeptide repeat , cephaloridine , escherichia coli , tetrapeptide , bacitracin , chemistry , microbiology and biotechnology , antibiotics , biochemistry , penicillin binding proteins , penicillin , biology , cephalosporin , enzyme , peptide , gene
Blocking peptidoglycan synthesis in Escherichia coli with moenomycin or vancomycin led to the accumulation of UDP‐MurNAc‐pentapeptide and of its immediate upstream precursors, whereas with cephaloridine or penicillin G the pool of UDP‐MurNAc‐pentapeptide decreased. With MraY and FtsW deficiencies the decrease of UDP‐MurNAc‐pentapeptide was accompanied by an increase of the upstream nucleotide precursors and the appearance of UDP‐MurNAc‐tetrapeptide.