Open AccessThe nematode Panagrellus redivivus is susceptible to killing by human pathogens at 37 °C
Author(s) -
Laws Thomas R.,
Smith Simon A.,
Smith Martin P.,
Harding Sarah V.,
Atkins Timothy P.,
Titball Richard W.
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.06.046
Subject(s) - biology , microbiology and biotechnology , caenorhabditis elegans , bacteria , yersinia pseudotuberculosis , burkholderia , yersinia , salmonella enterica , salmonella , staphylococcus aureus , nematode , virulence , genetics , ecology , gene
Caenorhabditis elegans has been used as a host for the study of bacteria that cause disease in mammals. However, a significant limitation of the model is that C. elegans is not viable at 37 °C. We report that the gonochoristic nematode Panagrellus redivivus survives at 37 °C and maintains its life cycle at temperatures up to and including 31.5 °C. The C. elegans pathogens Pseudomonas aeruginosa , Salmonella enterica , Staphylococcus aureus , but not Yersinia pseudotuberculosis , reduced P. redivivus lifespan. Of four strains of Burkholderia multivorans tested, one reduced P. redivivus lifespan at both temperatures, one was avirulent at both temperatures and two strains reduced P. redivivus lifespan only at 37 °C. The mechanism by which one of these strains killed P. redivivus at 37 °C, but not at 25 °C, was investigated further. Killing required viable bacteria, did not involve bacterial invasion of tissues, is unlikely to be due to a diffusible, bacterial toxin and was not associated with increased numbers of live bacteria within the intestine of the worm. We believe B. multivorans may kill P. redivivus by a temperature‐regulated mechanism similar to B. pseudomallei killing of C. elegans .