Open AccessMetabolism of fatty acid in yeast: Characterisation of β‐oxidation and ultrastructural changes in the genus Sporidiobolus sp. cultivated on ricinoleic acid methyl ester
Author(s) -
Feron Gilles,
BlinPerrin Caroline,
Krasniewski Isabelle,
Mauvais Geneviève,
Lherminier Jeannine
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.06.045
Subject(s) - ricinoleic acid , biochemistry , catalase , yeast , biology , fatty acid , thiolase , oxidase test , peroxisome , metabolism , enzyme , cell wall , mitochondrion , gene , castor oil
Cell structure modifications and β‐oxidation induction were monitored in two strains of Sporidiobolus , Sp. Ruinenii and Sp. pararoseus after cultivation on ricinoleic acid methyl ester. Ultrastructural observations of the yeast before and after cultivation on fatty acid esters did not reveal major modifications in Sp. ruinenii . Unexpectedly, in Sp. pararoseus a proliferation of the mitochondrion was observed. After induction, Sp. ruinenii principally exhibited an increase in the activities of acyl‐CoA oxidase (ACO), hydroxyacyl‐CoA deshydrogenase (HAD), thiolase and catalase. In contrast, Sp. pararoseus lacked ACO and catalase activities, but an increase in acyl‐CoA deshydrogenase (ACDH) and enoyl‐CoA hydratase (ECH) activity was observed. These data suggest that in Sp. ruinenii , β‐oxidation is preferentially localized in the microbody, whereas in Sp. pararoseus it might be localized in the mitochondria.