Open AccessFirst isolation of Streptococcus downei from human dental plaques
Author(s) -
Yoo So Young,
Kim KwanJoong,
Lim SeongHoon,
Kim KwangWon,
Hwang HoKeel,
Min ByungMoo,
Choe SonJin,
Kook JoongKi
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.06.020
Subject(s) - microbiology and biotechnology , streptococcus mutans , isolation (microbiology) , biology , restriction fragment length polymorphism , polymerase chain reaction , streptococcus salivarius , dental plaque , 16s ribosomal rna , streptococcus , bacitracin , streptococcus mitis , agar , bacteria , gene , genetics , antibiotics
In this study, we isolated four bacterial strains grown on mitis‐salivarius sucrose bacitracin agar. The strains had similar biochemical characteristics to biotypes I or II of mutans streptococci. The four isolates were identified as Streptococcus downei by 16S rDNA and dextranase gene ( dex ) sequencing as well as polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) targeting dex . To our knowledge, this is the first report of the isolation and identification of S. downei from dental plaque in humans. The results suggest that S. downei can inhabit the human oral cavity.