Open AccessDifferential expression of the chsE gene encoding a chitin synthase of Aspergillus nidulans in response to developmental status and growth conditions
Author(s) -
Lee Jeong Im,
Yu Yeong Man,
Rho Yoo Mi,
Park Bum Chan,
Choi Jeong Hwan,
Park HeeMoon,
Maeng Pil Jae
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.06.006
Subject(s) - chitin synthase , aspergillus nidulans , biology , chitin , gene , gene expression , mycelium , aspergillus , biochemistry , genetics , botany , chitosan , mutant
Expression of chsE encoding one of the five chitin synthases of Aspergillus nidulans was analyzed. Expression of chsE was moderate in conidiophores, but somewhat weaker in vegetative mycelia. During sexual development, chsE was expressed strongly in young cleistothecia and hülle cells, but little in mature sexual structures. Deletion of chsE caused a significant decrease in the chitin content of the cell wall during early sexual development. Expression of chsE was increased by substituting glucose with lactose or by addition of 0.6 M KCl or NaCl, but affected little by substituting glucose with sodium acetate. Consequently, chsE was shown to have a mode of expression distinct from those of the other chitin synthase genes, chsA , chsB and chsC .