Open AccessRapid detection of a gfp‐marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery
Author(s) -
Zhang Chong,
Xing XinHui,
Lou Kai
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.05.051
Subject(s) - enterobacter aerogenes , green fluorescent protein , fluorophore , fluorescence , anaerobic exercise , chemistry , kanamycin , reporter gene , biohydrogen , microbiology and biotechnology , biology , biochemistry , hydrogen production , gene , gene expression , escherichia coli , catalysis , physiology , physics , quantum mechanics
A gfp ‐ and kanamycin‐resistance gene‐containing plasmid pUCGK was successfully constructed and transformed into Enterobacter aerogenes to develop a rapid GFP‐based method for quantifying the bacterial concentration under anaerobic conditions for production of biohydrogen. Since the use of GFP as a molecular reporter is restricted by its requirement for oxygen in the development of the fluorophore, fluorescence detection for the fluorescent E. aerogenes grown anaerobically for hydrogen production was performed by developing a method of aerobic fluorescence recovery (AFR) of the anaerobically expressed GFP. By using this AFR method, rapid and non‐disruptive cell quantification of E. aerogenes by fluorescence density was achieved for analyzing the hydrogen production process.