Open AccessEffect of heat‐shock and bile salts on protein synthesis of Bifidobacterium longum revealed by [ 35 S]methionine labelling and two‐dimensional gel electrophoresis
Author(s) -
Savijoki Kirsi,
Suokko Aki,
Palva Airi,
Valmu Leena,
Kalkkinen Nisse,
Varmanen Pekka
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.05.032
Subject(s) - bifidobacterium longum , groel , heat shock protein , gel electrophoresis , proteolysis , methionine , biochemistry , chemistry , two dimensional gel electrophoresis , electrophoresis , bifidobacterium , polyacrylamide gel electrophoresis , lysis , heat shock , hsp70 , chromatography , microbiology and biotechnology , biology , amino acid , proteomics , enzyme , fermentation , lactobacillus , escherichia coli , gene
Experimental conditions for efficient protein radiolabelling and two‐dimensional gel electrophoresis were developed for Bifidobacterium longum . Using these tools, protein synthesis in cells before and after heat‐shock and bile salts treatment was investigated. Following heat‐stress, 13 proteins were upregulated, of which HtrA, DnaK and GroEL were also moderately induced by bile salts, indicating close relationship between the heat and bile salts responses in bifidobacteria. Our work indicated that, as a consequence of prolonged heat‐stress, HtrA undergoes sequential modification and proteolysis, and that this mechanism could be employed by bifidobacteria to respond to heat‐stress.