Open AccessrecA gene expression in a streptomycete is mediated by the unusual C‐terminus of RecA protein
Author(s) -
Ahel Ivan,
Mikoc Andreja,
Gamulin Vera
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.05.030
Subject(s) - mutant , gene , biology , streptomyces , streptomycetaceae , gene expression , strain (injury) , escherichia coli , regulation of gene expression , function (biology) , bacteria , dna , genetics , microbiology and biotechnology , actinomycetales , anatomy
Streptomyces RecA proteins are characterized by a conserved, positively charged extension of unknown function appended at their C‐termini. To investigate the function of this element, we introduced the Streptomyces rimosus recA gene and its mutant form encoding the protein with a C‐terminal deletion into S. rimosus . Both transcript and protein levels were dramatically increased in the strain expressing the truncated gene compared to the strain bearing the wild‐type recA , indicating involvement of the characteristic C‐terminal extension in regulating the recA expression in Streptomyces . Considering that RecA acts as a major regulator of DNA damage response in bacteria, this mode of regulation is expected to have broader implications and significance that outreaches our current understanding of RecA autoregulation.