Open AccessThe expression of selected non‐ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron
Author(s) -
Reiber Kathrin,
Reeves Emer P.,
Neville Claire M.,
Winkler Robert,
Gebhardt Peter,
Kavanagh Kevin,
Doyle Sean
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.05.028
Subject(s) - siderophore , aspergillus fumigatus , biology , peptide , microbiology and biotechnology , biochemistry , ribosomal rna , gene , gene expression
Three non‐ribosomal peptide synthetase genes, termed sidD , sidC and sidE , have been identified in Aspergillus fumigatus . Gene expression analysis by RT‐PCR confirms that expression of both sidD and C was reduced by up to 90% under iron‐replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron‐limiting conditions corresponded to SidD (200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT‐ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.