Open AccessDevelopment of an autotrophic culture system for the in vitro mycorrhization of potato plantlets
Author(s) -
Voets Liesbeth,
Dupré de Boulois Hervé,
Renard Laurent,
Strullu DésiréGeorges,
Declerck Stéphane
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.05.025
Subject(s) - biology , autotroph , symbiosis , mycelium , fungus , botany , spore , shoot , micropropagation , mycorrhiza , arbuscular mycorrhiza , photosynthesis , glomeromycota , tissue culture , in vitro , bacteria , biochemistry , genetics
An autotrophic culture system was developed for the in vitro mycorrhization of potato plantlets. Roots of micropropagated plantlets were associated to an arbuscular mycorrhizal fungus under in vitro conditions, while shoots developed under open air conditions. Several thousand spores, an extensive extraradical mycelium and an abundant root colonization were obtained. Spores were able to colonize new plantlets under the same conditions. These results support the capacity of the autotrophic culture system to continuously culture arbuscular mycorrhizal fungi and may serve as a powerful tool to investigate various aspects of the symbiosis for which a source–sink relationship and photosynthetic active tissues are necessary.