Open AccessThe rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius
Author(s) -
Matthew Caroline Z.,
Darby Alistair C.,
Young Simon A.,
Hume Lorna H.,
Welburn Susan C.
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.05.024
Subject(s) - biology , isolation (microbiology) , endosymbiosis , 16s ribosomal rna , bacteria , insect , gene , microbiology and biotechnology , genetics , botany , plastid , chloroplast
Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus: Glossina ) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa is discussed.