Open AccessIdentification of a promoter for the vegetative insecticidal protein‐encoding gene vip3LB from Bacillus thuringiensis
Author(s) -
Mesrati Lobna Abdelkefi,
Tounsi Slim,
Kamoun Fakher,
Jaoua Samir
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.04.032
Subject(s) - bacillus thuringiensis , primer extension , gene , biology , bacillus subtilis , transcription (linguistics) , genetics , promoter , microbiology and biotechnology , sigma factor , nucleic acid sequence , gene expression , nucleotide , bacteria , linguistics , philosophy
The expression of vip3LB , one of the vegetative insecticidal protein‐encoding genes of Bacillus thuringiensis , was studied at the transcriptional level. By primer extension analysis, we have identified, for the first time, the transcription start point of vip3 ‐type gene. Upstream from vip3LB transcription start point, was found a nucleotide sequence partially homologous to the consensus sequence for the E σ E holoenzyme of B. subtilis . Thus, it was strongly suggested that the identified vip3 promoter was under the control of σ 35 ‐like enzyme, the B. thuringiensis homolog of σ E . The transcriptional activity from the promoter was detected at the vegetative stage of growth starting at mid‐log phase as well as during the middle stage of sporulation.