Open AccessIsolation of genes differentially expressed during the fruit body development of Pleurotus ostreatus by differential display of RAPD
Author(s) -
Sunagawa Masahide,
Magae Yumi
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.04.018
Subject(s) - pleurotus ostreatus , rapd , biology , isolation (microbiology) , gene , differential display , genetics , microbiology and biotechnology , botany , mushroom , gene expression , medicine , population , environmental health , genetic diversity
To analyze genes involved in fruit body development of Pleurotus ostreatus , mRNAs from three different developmental stages: i.e., vegetative mycelium, primordium, and mature fruit body, were isolated and reverse‐transcribed to cDNAs. One hundred and twenty random PCR amplifications were performed with the cDNAs, which generated 382, 394, 393 cDNA fragments from each developmental stage. From these fragments, four cDNA clones specifically expressed in primordium or mature fruit body were detected. Sequence analysis and database searches revealed significant similarity with triacylglycerol lipase, cytochrome P450 sterol 14 α‐demethylase and developmentally regulated genes of other fungi. Northern blot analyses confirmed that all of the four cDNAs were unexpressed in mycelium, thus stage‐specific genes for fruit body formation of P. ostreatus were successfully isolated.