Open AccessFluorescence in situ hybridisation (FISH) – the next generation
Author(s) -
Zwirglmaier Katrin
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.04.015
Subject(s) - in situ hybridisation , fish <actinopterygii> , in situ , fluorescence in situ hybridization , sensitivity (control systems) , polynucleotide , biology , fluorescence , signal (programming language) , computational biology , chemistry , microbiology and biotechnology , fishery , genetics , computer science , physics , optics , engineering , electronic engineering , organic chemistry , gene , chromosome , programming language
Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this problem by increasing signal intensity. This review summarises various approaches for signal amplification, focussing especially on two widely recognised varieties, tyramide signal amplification and multiply labelled polynucleotide probes. Furthermore, new applications for FISH are discussed, which arise from the increased sensitivity of the method.