Open AccessComprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates
Author(s) -
Omoe Katsuhiko,
Hu DongLiang,
TakahashiOmoe Hiromi,
Nakane Akio,
Shinagawa Kunihiro
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.04.007
Subject(s) - toxin , staphylococcus aureus , biology , microbiology and biotechnology , genotyping , genotype , gene , mobile genetic elements , multiplex polymerase chain reaction , genetics , polymerase chain reaction , bacteria , genome
We describe a comprehensive detection system for 18 kinds of classical and newly described staphylococcal superantigenic toxin genes using four sets of multiplex PCR. Superantigenic toxin genotyping of Staphylococcus aureus for 69 food poisoning isolates and 97 healthy human nasal swab isolates revealed 32 superantigenic toxin genotypes and showed that many S. aureus isolates harbored multiple toxin genes. Analysis of the relationship between toxin genotypes and toxin genes encoding profiles of mobile genetic elements suggests its possible role in determining superantigenic toxin genotypes in S. aureus as combinations of toxin gene‐encoding mobile genetic elements.