Open AccessPrecise excision and secondary transposition of Tn phoA in non‐motile mutants of a Salmonella enterica serovar Enteritidis clinical isolate
Author(s) -
Amy Maı?té,
VirlogeuxPayant Isabelle,
Bottreau Elisabeth,
Mompart Florence,
Pardon Pierre,
Velge Philippe
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.03.018
Subject(s) - salmonella enterica , transposable element , biology , transposon mutagenesis , transposition (logic) , transposase , mutant , kanamycin , genetics , microbiology and biotechnology , gene , salmonella enteritidis , mutagenesis , genome , salmonella , escherichia coli , bacteria , linguistics , philosophy
Mutagenesis with Tn phoA has been widely used in many bacteria. Here, we report the excision and secondary transposition of this transposon in three non‐motile ( fliC , fliF and motB ) mutants of Salmonella enterica serovar Enteritidis ( S. Enteritidis). Isolation of motile revertants showed that they were kanamycin resistant and conserved a copy of Tn phoA in their genome in an insertion site different from the initial one. They also expressed an intact flagella. Characterization of the motile revertant derived from the fliC mutant showed that Tn phoA excised precisely from the fliC gene, resulting in an equivalent amount of FliC secreted protein in the revertant compared to that of the wild‐type strain. These results show that Tn phoA mutants should be used with care and underline the value of using transposon derivatives lacking the transposase gene.