Open AccessIdentification of an essential glycoprotease in Staphylococcus aureus
Author(s) -
Zheng Li,
Yang Junshu,
Landwehr Christina,
Fan Frank,
Ji Yinduo
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.03.017
Subject(s) - staphylococcus aureus , antimicrobial , microbiology and biotechnology , biology , drug development , in vitro , staphylococcal infections , gene , bacteria , computational biology , drug , genetics , pharmacology
The emergence of multi‐drug resistant bacterial pathogens is generating enormous public health concern, and highlights an urgent need for new, alternative agents for treating multi‐drug‐resistant pathogens. The gene products essential for bacterial growth in vitro and survival during infection constitute an initial set of protein targets for the development of antibacterial agents. In this study, we employed regulated gene expression approaches and demonstrated that a putative glycoprotease (Gcp) is required for staphylococcal growth in the culture. We found that Staphylococcus aureus becomes more sensitive to the Zn 2+ ion under the downregulation of Gcp expression in vitro. Bioinformatic analyses demonstrated that Gcp is conserved in many Gram‐positive pathogens and exists in a variety of Gram‐negative pathogens. Our results indicate that Gcp is a potential novel target for the development of antimicrobials against S. aureus infection.