Open AccessDiversity of carbazole‐degrading bacteria having the car gene cluster: Isolation of a novel gram‐positive carbazole‐degrading bacterium
Author(s) -
Inoue Kengo,
Habe Hiroshi,
Yamane Hisakazu,
Omori Toshio,
Nojiri Hideaki
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.03.009
Subject(s) - biology , sphingomonas , gene , bacteria , strain (injury) , gene cluster , microbiology and biotechnology , 16s ribosomal rna , pseudomonas , genetics , southern blot , anatomy
Twenty‐seven carbazole‐utilizing bacterial strains were isolated from environmental samples, and were classified into 14 groups by amplified ribosomal DNA restriction analysis. Southern hybridization analyses showed that 3 and 17 isolates possessed the car gene homologs of Pseudomonas resinovorans CA10 and Sphingomonas sp. strain KA1, respectively. Of the 17 isolates, 2 isolates also have the homolog of the carAa gene of Sphingomonas sp. strain CB3. While the genome of one isolate, a Gram‐positive Nocardioides sp. strain IC177, showed no hybridization to any car gene probes, PCR and sequence analyses indicated that strain IC177 had tandemly linked carAa and carC gene homologs whose deduced amino acid sequences showed 51% and 36% identities with those of strain KA1.