Open AccessGenetic modification of essential fatty acids biosynthesis in Hansenula polymorpha
Author(s) -
Laoteng Kobkul,
Ruenwai Rawisara,
Tanticharoen Morakot,
Cheevadhanarak Supapon
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.03.006
Subject(s) - biosynthesis , biochemistry , chemistry , biology , gene
The Δ 6 ‐desaturase gene isoform II involved in the formation of γ‐linolenic acid (GLA) was identified from Mucor rouxii . To study the possibility of alteration of the synthetic pathway of essential fatty acids in the methylotrophic yeast, Hansenula polymorpha , the cloned gene of M. rouxii under the control of the methanol oxidase ( MOX ) promoter of H. polymorpha , was used for genetic modification of this yeast. Changes in flux through the n ‐3 and n ‐6 pathways in the transgenic yeast were observed. The proportion of GLA varied dramatically depending on the growth temperature and media composition. This can be explained by the effects of either substrate availability or enzymatic activity. In addition to the potential application for manipulating the fatty acid profile, this study provides an attractive model system of H. polymorpha for investigating the deviation of fatty acid metabolism in eukaryotes.