Open AccessA critical step for relative quantification of mRNAs is selecting the correct internal controls
Author(s) -
Vahaboglu Haluk
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.02.033
Subject(s) - efflux , pseudomonas aeruginosa , messenger rna , mutant , strain (injury) , gene , real time polymerase chain reaction , polymerase chain reaction , biology , microbiology and biotechnology , chemistry , bacteria , genetics , anatomy
Dear Editor,We read with great excitement the manuscript entitled “Measurement of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real-time polymerase chain reaction” by Yoneda et al. [1]. The authors described a relative quantitation assay using a real time PCR (RT-PCR) method for multi-drug efflux proteins, MexB and MexY, in several clinical strains of P. aeruginosa relative to a reference strain PAO1. Some isogenic mutants of PAO1 were also compared. The relative expression of mRNAs were further validated by comparison to corresponding protein levels. The rpsL gene provided the internal control.The authors stated that the mRNA levels of the isogenic mutants agreed well with those of strain PAO1 and there were only slight discrepancies between mRNA and protein levels of clinical strains. …