Open AccessCTX‐M and SHV‐12 β‐lactamases are the most common extended‐spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 3 university hospitals within Korea
Author(s) -
Kim Jungmin,
Lim YuMi,
Rheem Insoo,
Lee Yeonhee,
Lee JeChul,
Seol SungYong,
Lee YuChul,
Cho DongTaek
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.02.029
Subject(s) - klebsiella pneumoniae , microbiology and biotechnology , escherichia coli , kanamycin , tobramycin , biology , gentamicin , tetracycline , plasmid , klebsiella , enterobacteriaceae , streptomycin , antibiotics , gene , genetics
Among the 443 clinical isolates of Escherichia coli and Klebsiella spp. collected between June and November 2003 from 3 university hospitals in Korea, 62 isolates were confirmed as extended‐spectrum β‐lactamase (ESBL)‐ or plasmid‐mediated AmpC β‐lactamase‐producers by double disk synergy test, PCR and sequencing for β‐lactamase genes. The most frequently identified ESBL gene among E. coli and K. pneumoniae isolates was bla SHV‐12 and bla CTX‐M ( bla CTX‐M‐9 , bla CTX‐M‐14 , bla CTX‐M‐3 , and bla CTX‐M‐15 ). Four kinds of plasmid‐mediated AmpC β‐lactamases, ACT‐1, CMY‐1, CMY‐2, and DHA‐1, were detected. ESBL production was associated with high levels of resistance to tetracycline, sulfisoxazole, streptomycin, kanamycin, gentamicin and tobramycin when compared to non‐ESBL producing isolates. Conclusively, this study suggests that the CTX‐M β‐lactamases are prevalent and various kinds of plasmid‐mediated AmpC enzymes are distributed in clinical isolates of E. coli and Klebsiella spp. in Korea.