Open AccessDNA array analysis of Candida albicans gene expression in response to adherence to polystyrene
Author(s) -
Marchais Véronique,
Kempf Marie,
Licznar Patricia,
Lefrançois Corinne,
Bouchara JeanPhilippe,
Robert Raymond,
Cottin Jane
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.02.014
Subject(s) - candida albicans , gene , germ tube , transcriptome , biology , gene expression , dna , corpus albicans , microbiology and biotechnology , biochemistry
Candidiasis is often initiated by the colonization of inert surfaces. In order to elucidate the mechanisms involved in this adherence process, DNA macroarrays were used to analyze the transcriptome of Candida albicans , the main causative agent of this mycoses, in a simple adherence model using germ tubes produced in polystyrene Petri dishes. Non‐adherent germ tubes produced on glass surface were used as a control. Analysis of gene expression displayed 77 genes identified as statistically overexpressed in adherent germ tubes. Among these genes, some encoded enzymes participating in metabolism of lipids (such as LIP6 ), of proteins (such as SAP1 ) or of carbohydrates (like PGI1 , PMI40 and PSA1 . Some of these genes have already been reported as playing a role in pathogenesis of C. albicans . However, functions were unknown for a large part (45.5%) of the overexpressed genes which will be analyzed further in order to define their relationship with adherence.