Open AccessGenotyping of Chromobacterium violaceum isolates by recA PCR‐RFLP analysis
Author(s) -
Scholz Holger Christian,
Witte Angela,
Tomaso Herbert,
Al Dahouk Sascha,
Neubauer Heinrich
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.02.005
Subject(s) - genotyping , chromobacterium violaceum , restriction fragment length polymorphism , biology , genetics , polymerase chain reaction , genotype , microbiology and biotechnology , gene , virulence , quorum sensing
Intraspecies variation of Chromobacterium violaceum was examined by comparative sequence – and by restriction fragment length polymorphism analysis of the recombinase A gene ( recA ‐PCR‐RFLP). Primers deduced from the known recA gene sequence of the type strain C. violaceum ATCC 12472 T allowed the specific amplification of a 1040 bp recA fragment from each of the 13 C. violaceum strains investigated, whereas other closely related organisms tested negative. Hin dII– Pst I– recA RFLP analysis generated from 13 representative C. violaceum strains enabled us to identify at least three different genospecies. In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence‐based approach to specifically identify and classify C. violaceum on genospecies level.