Open AccessRecX is involved in antioxidant mechanisms of the radioresistant bacterium Deinococcus radiodurans
Author(s) -
Sheng Duohong,
Gao Guanjun,
Tian Bing,
Xu Zhenjian,
Zheng Zhiguo,
Hua Yuejin
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.01.051
Subject(s) - deinococcus radiodurans , superoxide dismutase , catalase , reactive oxygen species , antioxidant , radioresistance , deinococcus , bacteria , biology , mutagenesis , biochemistry , microbiology and biotechnology , chemistry , genetics , gene , mutation , cell culture
Deinococcus radiodurans shows remarkable resistance to reactive oxygen species (ROS), generated by irradiation. Disruption of recX (dr1310) in D. radiodurans using targeted mutagenesis method enhanced its ROS scavenging activity, and recX overexpression in this bacterium repressed its antioxidant activity significantly. Further analyses on catalase and superoxide dismutase, two important antioxidant proteins in cells, showed that RecX could repress the induction of antioxidant enzymes, revealing that it negatively regulates the ROS scavenging activity in D. radiodurans .