Open AccessIdentification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA
Author(s) -
Levy Haim,
Fisher Morly,
Ariel Naomi,
Altboum Zeev,
Kobiler David
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.01.039
Subject(s) - rapd , bacillus anthracis , biology , strain (injury) , genetic marker , genotype , genetics , dna , polymerase chain reaction , microbiology and biotechnology , bacteria , gene , genetic diversity , population , medicine , environmental health , anatomy
Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method – Random Amplification of Polymorphic DNA (RAPD) – to identify genetic markers in B. anthracis strains. Twenty‐five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD‐markers were cloned and sequenced. The five RAPD‐derived genotypes could be defined by integration of these three markers. This system offers a simple non‐expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium.