Open AccessDevelopment of a generic PCR detection of deoxynivalenol‐ and nivalenol‐chemotypes of Fusarium graminearum
Author(s) -
Li HePing,
Wu AiBo,
Zhao ChunSen,
Scholten Olga,
Löffler Huub,
Liao YuCai
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.01.015
Subject(s) - chemotype , fusarium , mycotoxin , biology , trichothecene , microbiology and biotechnology , intergenic region , gene , botany , genetics , genome , essential oil
Based on the intergenic sequences of Tri5–Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)‐chemotypes and a 360 bp sequence of nivalenol (NIV)‐ chemotypes of Fusarium graminearum . Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON‐chemotypes and 54 were NIV‐chemotypes. Sequence analyses revealed that DON‐chemotypes display more variations than NIV‐chemotypes. This PCR assay could be used to detect mycotoxin‐producing Fusarium ‐species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody‐based mycotoxin tests.