Open AccessPre‐exposure to UV irradiation increases the transfer frequency of the IncJ conjugative transposon‐like elements R391, R392, R705, R706, R997 and pMERPH and is recA + dependent
Author(s) -
McGrath Barry M.,
O'Halloran John A.,
Pembroke J. Tony
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.01.013
Subject(s) - transposable element , irradiation , chemistry , dna transposable elements , biology , genetics , physics , mutant , gene , nuclear physics
The enteric conjugative transposon‐like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10 −5 to 10 −3 per recipient, upon pre‐exposure to UV irradiation. However, the transfer frequency of R997, which was higher than the other IncJ elements at 10 −3 per donor, showed a smaller increase. This effect was shown to be recA + dependent in all cases. Using PCR primers directed outwards from the ends of the integrated R391 element it was observed that a circular intermediate of the element forms within the host, which has been proposed to be a transfer intermediate. Using real‐time PCR, it was determined that the amount of the circular intermediate produced increased substantially upon UV irradiation.