Open AccessAssessment of a new technique combining a viability test, whole‐cell hybridization and laser‐scanning cytometry for the direct counting of viable Enterobacteriaceae cells in drinking water
Author(s) -
Baudart Julia,
Olaizola Amandine,
Coallier Josée,
Gauthier Vincent,
Laurent Patrick
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2005.01.001
Subject(s) - enterobacteriaceae , enumeration , microbiology and biotechnology , flow cytometry , cytometry , fish <actinopterygii> , biology , escherichia coli , fishery , biochemistry , mathematics , combinatorics , gene
A new direct approach, called direct viable count (DVC)‐FISH‐ScanRDI, combining viability measurement, specific detection and sensitive enumeration of highly diluted Enterobacteriaceae cells, was assessed during the summer in water samples from a North American drinking water treatment plant and its distribution system. Major results of this field investigation show a higher sensitivity of the DVC–FISH–ScanRDI approach in enumerating viable Enterobacteriaceae cells in distributed drinking water, relative to a culture‐based method, and the increased concentration of viable but non‐culturable (VBNC) Enterobacteriaceae cells in distributed water for temperatures above 18 °C.