Open AccessSensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop‐mediated isothermal amplification method
Author(s) -
Song Tianyan,
Toma Claudia,
Nakasone Noboru,
Iwanaga Masaaki
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.12.014
Subject(s) - loop mediated isothermal amplification , shigella , escherichia coli , microbiology and biotechnology , bacteria , enterobacteriaceae , biology , escherichia , gene , genetics , dna
Here we report a loop‐mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli . The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.