Open AccessCharacterization of outer membrane protein fractions of Bdellovibrionales
Author(s) -
Beck Sebastian,
Schwudke Dominik,
Appel Bernd,
Linscheid Michael,
Strauch Eckhard
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.12.006
Subject(s) - bacterial outer membrane , chemistry , biochemistry , escherichia coli , gene
Bdellovibrio ‐and‐like organisms (BALOs) are predatory bacteria that prey upon Gram‐negative bacteria and are taxonomically subsumed in the order Bdellovibrionales . Despite their unique lifestyle, these bacteria show remarkable genotypic diversities. The outer membrane of the predators is likely to play an important role during the recognition and invasion stage, as well as in the intraperiplasmic growth phase. In this study, the outer membrane protein fractions of type strains of Bdellovibrio , Bacteriovorax and Peredibacter were investigated, revealing the presence of outer membrane proteins (Omps) similar to the major Omps of Bdellovibrio bacteriovorus . The primary structures of these Omps of Bdellovibrio sp. W, Bacteriovorax stolpii and Peredibacter starrii were elucidated by a combined mass spectrometric–reverse genetic approach. The similarity between the analyzed Omps of the investigated BALOs ranges from 32% to 89% showing conserved amino acid regions in their primary structure.