Open AccessMeasurement of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real‐time polymerase chain reaction
Author(s) -
Yoneda Kazuhiko,
Chikumi Hiroki,
Murata Takeshi,
Gotoh Naomasa,
Yamamoto Hiroyuki,
Fujiwara Hiromitsu,
Nishino Takeshi,
Shimizu Eiji
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.11.048
Subject(s) - efflux , pseudomonas aeruginosa , multiple drug resistance , real time polymerase chain reaction , polymerase chain reaction , messenger rna , blot , biology , microbiology and biotechnology , antibiotics , gene expression , bacteria , chemistry , gene , biochemistry , genetics
Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa . Pump expression usually has been quantified by Western blotting. Quantitative real‐time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump protein quantities is unclear. We devised a real‐time PCR for mRNA expression of MexAB‐OprM and MexXY‐OprM multidrug efflux pumps. In laboratory strains differing in MexB and MexY expression and in several clinical isolates, protein and mRNA expression correlated well. Quantitative real‐time PCR should be a useful alternative in quantitating expression of multidrug efflux pumps by P. aeruginosa isolates in clinical laboratories.