Open AccessIdentification and characterization of a cell‐wall anchored DNase gene in Clostridium perfringens
Author(s) -
Okumura Kayo,
Kawsar Hameem I.,
Shimizu Takeshi,
Ohta Toshiko,
Hayashi Hideo,
Shimizu Tohru
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.11.019
Subject(s) - clostridium perfringens , gene , mutant , nuclease , signal peptide , biology , deoxyribonuclease i , sequence analysis , microbiology and biotechnology , recombinant dna , biochemistry , genetics , bacteria , base sequence
Completion of the whole genome sequence of Clostridium perfringens strain 13 revealed the presence of an extracellular nuclease gene, cadA . Transcriptional analysis showed that the cadA gene is negatively regulated by the two‐component VirR/VirS system and its secondary regulator VR‐RNA. The CadA protein possesses an N‐terminal signal sequence and a Gram‐positive cell wall anchoring motif consisting of a sorting signal (LPXTG motif), a hydrophobic domain, and positively charged residues at the end of C‐terminus. By comparing the DNase production between the wild type and the cadA mutant, and DNase activity assay with the recombinant truncated CadA protein, we confirmed that the cadA gene product is one of the DNases produced by C. perfringens .