Open AccessA pore‐forming toxin produced by Aeromonas sobria activates cAMP‐dependent Cl − secretory pathways to cause diarrhea
Author(s) -
Tanoue Naomi,
Takahashi Akira,
Okamoto Keinosuke,
Fujii Yoshio,
Taketani Yutaka,
Harada Nagakatsu,
Nakano Masayuki,
Nakaya Yutaka
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.11.009
Subject(s) - efflux , intracellular , microbiology and biotechnology , chemistry , diarrhea , secretion , biology , biochemistry , medicine
Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria , a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl − transport in human colonic epithelial cells. ASH increased short‐circuit currents (Isc) and 125 I efflux from Caco‐2 cells, indicating ASH activate Cl − secretion. Additions of inhibitors of cyclic AMP dependent Cl − channels, glybenclamide and NPPB suppressed the Isc and 125 I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP‐dependent Cl − secretory pathway could be related with diarrhea induced by A. sobria .