Open AccessAspergillus oryzae produces compounds inhibiting cholesterol biosynthesis downstream of dihydrolanosterol
Author(s) -
Hajjaj Hassan,
Duboc Philippe,
Fay Laurent B.,
Zbinden Irène,
Macé Katherine,
Niederberger Peter
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.11.001
Subject(s) - aspergillus oryzae , lanosterol , biosynthesis , chemistry , mevalonic acid , in vitro , biochemistry , cholesterol , enzyme , lathosterol , mevalonate pathway , aspergillus , sterol , biology , microbiology and biotechnology , campesterol
The formation of cholesterol synthesis inhibiting molecules by five different strains of the koji mold Aspergillus oryzae was studied. After growing these strains on a complex liquid medium we found in crude organic phase extracts and specific fractions there from compounds inhibiting cholesterol synthesis in human hepatic T9A4 cells in vitro at enzyme sites downstream of dihydrolanosterol. This was evidenced by using different radioactively labeled precursors, namely acetate, mevalonate, 24,25‐dihydro‐[24,25‐ 3 H 2 ]‐lanosterol or [3‐ 3 H]‐lathosterol.