Open AccessPHB synthase from Streptomyces aureofaciens NRRL 2209
Author(s) -
Ramachander T.V.N.,
Rawal S.K.
Publication year - 2005
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.10.034
Subject(s) - streptomyces aureofaciens , atp synthase , chemistry , streptomyces , microbiology and biotechnology , biochemistry , stereochemistry , biology , bacteria , enzyme , genetics
An ∼4.9 kb Sau3A I genomic DNA fragment from the Streptomyces aureofaciens NRRL 2209 aiding in the biosynthesis of PHB in recombinant Escherichia coli has been sequenced and analysed for phaC gene. The putative phaC Sa gene of 2 kb is 79.1% GC rich and encodes a 63.5 kDa protein. It expressed under its own promoter and significant PHA synthase activity was detected in the recombinant E. coli . This is the first putative PHA synthase gene reported from a Streptomyces sp. with serine as the active nucleophile in the conserved lipase box. The phaC Sa was found in close proximity to a regulatory gene, which apparently regulated the phaC expression.