Open AccessBxz1, a new generalized transducing phage for mycobacteria
Author(s) -
Lee Sunhee,
Kriakov Jordan,
Vilcheze Catherine,
Dai Zhiyan,
Hatfull Graham F.,
Jacobs William R.
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.10.032
Subject(s) - mycobacterium smegmatis , transposable element , plasmid , biology , mycobacterium , point mutation , microbiology and biotechnology , genetics , gene , mutation , mutant , bacteria , mycobacterium tuberculosis , tuberculosis , medicine , pathology
We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae . Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10 −8 to 10 −6 per plaque forming unit between strains of M. smegmatis . We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene.