Open AccessTemporal temperature gradient gel electrophoresis (TTGE) as a tool for the characterization of arbuscular mycorrhizal fungi
Author(s) -
Cornejo Pablo,
AzcónAguilar Concepción,
Miguel Barea José,
Ferrol Nuria
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.10.030
Subject(s) - glomus , biology , arbuscular mycorrhizal , temperature gradient gel electrophoresis , botany , mycorrhiza , 18s ribosomal rna , nested polymerase chain reaction , ribosomal rna , 16s ribosomal rna , symbiosis , polymerase chain reaction , gene , spore , bacteria , genetics
The aim of this study was to assess the feasibility of using temporal temperature gradient electrophoresis (TTGE) of PCR‐amplified 18S rDNA fragments of different Glomus species for their detection and characterization. Screening of Glomus clarum , Glomus constrictum , Glomus coronatum , Glomus intraradices , Glomus mosseae and Glomus viscosum by PCR‐TGGE revealed that the NS31‐AM1 region of the 18S rRNA gene contained insufficient variation to discriminate between them. In contrast, TTGE analysis of the NS31‐Glo1 region, which was obtained by nested PCR of the NS31‐AM1 amplicon, showed that each species was characterized by a specific TTGE fingerprint. However, isolates of the same species could not be distinguished. The nested PCR‐TTGE approach developed allowed identification of the Glomus species colonising the roots of different plant species.