Open AccessRapid detection of Mycoplasma dispar and M. bovirhinis using allele specific polymerase chain reaction protocols
Author(s) -
Miles Katie,
McAuliffe Laura,
Ayling Roger D.,
Nicholas Robin A.J.
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.10.010
Subject(s) - polymerase chain reaction , biology , oligonucleotide , mycoplasma , ribosomal rna , genetics , 16s ribosomal rna , allele , ribosomal dna , microbiology and biotechnology , nucleic acid sequence , dna , gene , phylogenetics
We describe an allele specific PCR based approach for the rapid detection of two bovine Mycoplasma species associated with respiratory disease. Specific and universal oligonucleotides were used in combination to detect the presence of single nucleotide polymorphisms within the 16S ribosomal DNA sequence. Presence of Mycoplasma 16S rDNA is indicated by the production of a single control fragment, whilst positive samples generate an alternative smaller specific product over the same region. This technique provides a reliable and sensitive method which, although widely used in human genetic screening, has not been documented for diagnosis of bacterial infection.