Open AccessIdentification, distribution pattern of IS 231 elements in Bacillus thuringiensis and their phylogenetic analysis
Author(s) -
Huang Tianpei,
Liu Jingjing,
Song Fuping,
Shu Changlong,
Qiu Junzhi,
Guan Xiong,
Huang Dafang,
Zhang Jie
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.09.037
Subject(s) - bacillus thuringiensis , phylogenetic tree , identification (biology) , biology , bacillales , distribution (mathematics) , evolutionary biology , computational biology , genetics , botany , bacteria , mathematics , gene , mathematical analysis , bacillus subtilis
In order to better understand the fundamental biology of Bacillus thuringiensis , a single oligonucleotide primer (5′‐CATSSCCATCAASYTAAVR‐3′) was used to investigate the distribution pattern of IS 231 elements in B. thuringiensis by PCR. The results indicated that IS 231 elements appeared in 20 standard strains and 107 of 111 China isolates. Three novel IS 231 , IS 231 J, IS 231 O and IS 231 Q, five variants and a mobile insertion cassette MICBth 4 were cloned from eight standard strains of B. thuringiensis , respectively. Interestingly, BLAST analysis revealed that the 5′ end of novel IS 231 J shared 99% identity in 495‐bp with a DNA segment adjacent to the 3′ end of B. thuringiensis vip1Ac gene (GenBank Accession No. AY245547). Two phylogenetic trees of IS 231 elements were constructed and analyzed by neighbor‐joining and UPGMA methods from PHYLIP 3.6b program, respectively.