Open AccessRapid detection of Panton–Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real‐time PCR
Author(s) -
Deurenberg Ruud H.,
Vink Cornelis,
Driessen Christel,
Bes Michèle,
London Nancy,
Etienne Jerome,
Stobberingh Ellen E.
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.09.031
Subject(s) - staphylococcus aureus , panton–valentine leukocidin , leukocidin , microbiology and biotechnology , biology , micrococcaceae , polymerase chain reaction , virology , bacteria , methicillin resistant staphylococcus aureus , gene , genetics
To allow rapid identification of Panton–Valentine leukocidin (PVL)‐producing Staphylococcus aureus strains, a real‐time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL‐positive S. aureus strains, and neither with PVL‐negative strains nor staphylococci other than S. aureus .