Open AccessRapid identification of probiotic Lactobacillus species by multiplex PCR using species‐specific primers based on the region extending from 16S rRNA through 23S rRNA
Author(s) -
Kwon HyukSang,
Yang EunHee,
Yeon SeungWoo,
Kang ByoungHwa,
Kim TaeYong
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.08.049
Subject(s) - lactobacillus gasseri , lactobacillus casei , biology , probiotic , lactobacillus , lactobacillus rhamnosus , lactobacillus reuteri , 16s ribosomal rna , 23s ribosomal rna , microbiology and biotechnology , primer (cosmetics) , multiplex polymerase chain reaction , lactobacillus acidophilus , ribosomal rna , lactobacillus plantarum , polymerase chain reaction , genetics , bacteria , gene , chemistry , lactic acid , ribosome , organic chemistry , rna
This study aimed to develop a novel multiplex polymerase chain reaction (PCR) primer set for the identification of seven probiotic Lactobacillus species such as Lactobacillus acidophilus , Lactobacillus delbrueckii , Lactobacillus casei , Lactobacillus gasseri , Lactobacillus plantarum , Lactobacillus reuteri and Lactobacillus rhamnosus . The primer set, comprising of seven specific and two conserved primers, was derived from the integrated sequences of 16S and 23S rRNA genes and their rRNA intergenic spacer region of each species. It was able to identify the seven target species with 93.6% accuracy, which exceeds that of the general biochemical methods. The phylogenetic analyses, using 16S rDNA sequences of the probiotic isolates, also provided further support that the results from the multiplex PCR assay were trustworthy. Taken together, we suggest that the multiplex primer set is an efficient tool for simple, rapid and reliable identification of seven Lactobacillus species.