Open AccessThe Aspergillus niger annexin, anxC3.1 is constitutively expressed and is not essential for protein secretion
Author(s) -
Khalaj Vahid,
Hey Peter,
Smith Lyndsay,
Robson Geoffrey D.,
Brookman Jayne
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.08.034
Subject(s) - secretion , annexin , aspergillus niger , biology , gene , biochemistry , heat shock protein , gene expression , secretory protein , messenger rna , microbiology and biotechnology , in vitro
An annexin, anxC3.1 , was isolated and characterised from the industrially important filamentous fungus Aspergillus niger . anxC3.1 is a single copy gene encoding a 506 amino acid predicted protein which contains four annexin repeats. Disruption of the anxC3.1 gene did not lead to any visible changes in phenotype, nor in the levels of secreted protein, nor specifically in glucoamylase production, suggesting no major role in secretion. anxC3.1 expression was found to be unaltered under a variety of conditions such as increased secretion, altered nitrogen source, heat shock, and decreased Ca 2+ levels, indicating that anxC3.1 is constitutively expressed. This is the first reported functional characterisation of a fungal annexin.