Open AccessA xylose‐inducible expression system for Lactococcus lactis
Author(s) -
Miyoshi Anderson,
Jamet Emmanuel,
Commissaire Jacqueline,
Renault Pierre,
Langella Philippe,
Azevedo Vasco
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.08.018
Subject(s) - lactococcus lactis , xylose , cytoplasm , lactococcus , heterologous , extracellular , signal peptide , biology , heterologous expression , biochemistry , microbiology and biotechnology , gene , chemistry , bacteria , fermentation , recombinant dna , genetics , lactic acid
A new controlled production system to target heterologous proteins to cytoplasm or extracellular medium is described for Lactococcus lactis NCDO2118. It is based on the use of a xylose‐inducible lactococcal promoter, P xylT . The capacities of this system to produce cytoplasmic and secreted proteins were tested using the Staphylococcus aureus nuclease gene ( nuc ) fused or not to the lactococcal Usp45 signal peptide. Xylose‐inducible nuc expression is tightly controlled and resulted in high‐level and long‐term protein production, and correct targeting either to the cytoplasm or to the extracellular medium. Furthermore, this expression system is versatile and can be switched on or off easily by adding either xylose or glucose, respectively. These results confirm the potential of this expression system as an alternative and useful tool for the production of proteins of interest in L. lactis .