Open AccessIntragenomic heterogeneity of the 16S rRNA–23S rRNA internal transcribed spacer among Pseudomonas syringae and Pseudomonas fluorescens strains
Author(s) -
Milyutina Irina A.,
Bobrova Vera K.,
Matveeva Eugenia V.,
Schaad Norman W.,
Troitsky Alexey V.
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.08.010
Subject(s) - internal transcribed spacer , biology , pseudomonas fluorescens , 23s ribosomal rna , genetics , ribosomal rna , spacer dna , pseudomonas syringae , horizontal gene transfer , genome , gene , bacteria , rna , ribosome
The 16S–23S rRNA internal transcribed spacer regions (ITS1) from 14 strains of Pseudomonas syringae and P. fluorescens were sequenced. ITS1 exhibited significant sequence variability among different operons within a single genome. From 1 to 4 types of ITS1 were found in individual genomes of the P. syringae and P. fluorescens strains. A total of eight ITS1 types were identified among strains studied. The ITS1 nucleotide sequences consisted of conserved blocks including, among others, a stem‐forming region of box B, tRNA Ile and tRNA Ala genes and several variable blocks. The differences in the variable regions were mostly due to insertions and/or deletions of nucleotide blocks. The intragenomic heterogeneity of ITS1 was brought about by different combinations of variable blocks, which possibly have resulted from recombination and horizontal transfer.