Open AccessPseudomonas fluorescens infection by bacteriophage ΦS1: the influence of temperature, host growth phase and media
Author(s) -
Sillankorva Sanna,
Oliveira Rosário,
Vieira Maria João,
Sutherland Ian,
Azeredo Joana
Publication year - 2004
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1016/j.femsle.2004.06.058
Subject(s) - pseudomonas fluorescens , bacteriophage , lysis , host (biology) , microbiology and biotechnology , biology , bacteria , pseudomonas , biochemistry , genetics , escherichia coli , gene
The influence of host growth temperature, phase and media, together with the effect of infection temperature on bacteriophage ΦS1 infection of Pseudomonas fluorescens were examined. The rates of cell lysis and phage release were determined and showed that the efficacy of phage infection was optimal with host cells grown and infected at 26 °C. The host physiological state also affected these rates. Infection was dependent on the presence of cell wall proteins with molecular weights of 17.5 ± 1 and 99 ± 5 kDa.