Aggregation of mycobacteria caused by disruption of fibronectin-attachment protein-encoding gene | Zendy

Y MIYAMOTO | Zendy

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Aggregation of mycobacteria caused by disruption of fibronectin-attachment protein-encoding gene

Author(s) -

Y MIYAMOTO

Publication year - 2004

Publication title -

fems microbiology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.899

H-Index - 151

eISSN - 1574-6968

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2004.05.047

Subject(s) - mycobacterium smegmatis , complementation , gene , fibronectin , internalization , biology , bacteria , mutant , microbiology and biotechnology , sortase , cell , genetics , mycobacterium tuberculosis , bacterial protein , tuberculosis , medicine , pathology

The fibronectin-attachment protein (FAP) is conserved among several species of mycobacteria. Although this protein is associated with attachment and internalization of bacteria to host cells via fibronectin, the physiological role of the protein still remains unclear. To investigate this point, we generated FAP gene disruptant in Mycobacterium smegmatis. The gene disruption, verified by Southern blot and PCR analysis, induced changes on the bacteria, which are associated with strong aggregation and alteration of cell surface properties. Increased hydrophobicity and Congo red accumulation was observed in the FAP gene disruptant. In addition, the complementation experiment demonstrated that the corresponding gene restored wild type morphology in the disruptant. These results indicate that the FAP affects the cell surface properties, and its deletion lead to enhanced aggregation of the M. smegmatis.

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