Development of PCR assay to identify Pseudomonas fluorescens and its biotype. | Zendy

Mauro Scarpellini | Zendy; L. Franzetti | Zendy; Antoniëtta Galli | Zendy

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Development of PCR assay to identify Pseudomonas fluorescens and its biotype.

Author(s) -

Mauro Scarpellini,

L. Franzetti,

Antoniëtta Galli

Publication year - 2004

Publication title -

pubmed

Language(s) - English

Resource type - Journals

pISSN - 0378-1097

DOI - 10.1016/j.femsle.2004.05.043

Subject(s) - haeiii , pseudomonas fluorescens , biology , 16s ribosomal rna , polymerase chain reaction , microbiology and biotechnology , pseudomonas , restriction enzyme , restriction fragment length polymorphism , genetics , gene , bacteria

The paper provides a simple protocol that uses the polymerase chain reaction to amplify a specific portion of the 16S gene, allowing the recognition of Pseudomonas fluorescens from other group I Pseudomonas. The amplified DNA patterns of 16S rRNA and ITS1, from the restriction fragment length polymorphisms VspI, HaeIII and TaqI digestion, produced band patterns that distinguished the biotypes of Ps. fluorescens. In addition to distinguishing the biotypes C and 3 we used a phenotypical method for levan production.

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